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pRS424
pRS424
規(guī)格:
貨期:
編號:B161414
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRS424
商品貨號 B161414
Designations pRS424
Depositors TW Christianson
Biosafety Level 1
Vector Information
Size (kb): 5.616
Vector: pRS424 (phagemid)
Promoters: Promoter T7 (phi10)
Construction: pJS92, pRS304, pBluescript II SK+
Marker(s):TRP1,ampR
Construct size (kb): 5.616000175476074
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: T3
promoter: T7 (phi10)
promoter: lac
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; PvuII--5.2, 0.5; XbaI--3.3, 1.4, 1.0.
Contains the REP3 and FRT sequences necessary for high copy propagation in yeast.
In S. cerevisiae, the number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 1.5 + or - 1.7% of progeny per doubling.
YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.
The order of the major features in this plasmid is: ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori(2 micron) - TRP1.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568

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