| 產(chǎn)品名稱 |
DS-1 |
| 商品貨號 |
B164371 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with canine ASP in immunoprecipitation, ELISA and Western Blot assays. The antibody cross reacts with human ASP. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against alveolar surfactant protein (ASP) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against alveolar surfactant protein (ASP) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with canine alveolar surfactant proteins. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with canine ASP in immunoprecipitation, ELISA and Western Blot assays. The antibody cross reacts with human ASP. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG |
| Name of Depositor |
California Biotechnology Inc. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Lewicki J. Monoclonal antibodies against alveolar surfactant protein. US Patent 4,656,253 dated Apr 7 1987
|
